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KMID : 0613820090190081164
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Journal of Life Science
2009 Volume.19 No. 8 p.1164 ~ p.1169
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5-Aza-2’-deoxycytidine Inhibits the Maintenance of Cancer Stem Cell in a Mouse Model of Breast Cancer
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Nho Kyoung-Jin
Yang In-Sook
Kim Ran-Ju
Kim Soo-Rim
Park Jeong-Ran
Jung Ji-Youn
Cho Sung-Dae
Nam Jeong-Seok
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Abstract
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Aberrant DNA methylation plays an important role in the development of cancer. It has been reported recently that DNA hypermethylation is involved in the maintenance of cancer stem cells. The present study was designed to test the hypothesis that the demethylating agent, 5-aza-2‘-deoxycytidine (AZA), can inhibit the potential for maintenance of cancer stem cells. To validate this hypothesis, we used 4T1 syngeneic mouse models of breast cancer. The AZA pre-treated 4T1 cells showed a dramatic inhibition of tumorsphere formation, compared to their counterparts in vitro. In addition, the AZA treatment significantly suppressed the expression of stem regulator genes, such as oct-4, nanog and sox2, compared to counterparts in vivo. Therefore, selective inhibition of DNA methylation may be useful for stem-specific cancer therapy.
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KEYWORD
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5-aza-2’-deoxycytidine, DNA methylation, cancer stem cell, stem regulator gene
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